Efeito da suplementação com selênio e com as vitaminas C e E sobre biomarcadores hematológicos em militares durante treinamento físico vigoroso e prolongado
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Resumo
Neste estudo, investigamos o efeito da suplementação com selênio, vitaminas C e E sobre biomarcadores hematológicos em militares submetidos a exercício físico vigoroso e prolongado (EFVP). Participaram 24 homens (32,0 ± 7,2 anos), durante 37 dias (18 antes do treinamento e, 19 durante EFVP). Dois grupos foram separados aleatoriamente: Grupo Suplementado (GS) e Grupo Placebo (GP). O GS (n=13) ingeriu cápsulas contendo selênio quelado (0,06 mg), vitamina C (1000 mg) e vitamina E (500 mg) e, o GP: (n=11) ingeriu (1500 mg de amido). O sangue foi coletado antes do início do estudo (T0); ao final da primeira (T1) e terceira (T2) semana de EFVP. Os dados foram analisados pela ANOVA 3X2 (p<0,05). A hemoglobina reduziu em T1 e T2 no GS e GP versus T0. A hemoglobina corpuscular média reduziu em T1 e T2 no GS e no GP apenas em T2 versus T0. A concentração da hemoglobina corpuscular média reduziu no GS em T1 e T2 versus T0, porém no GP reduziu em T2 versus T0 e T1. As hemácias, hematócrito, volume corpuscular médio e RDW não apresentaram diferenças significativas. O EFVP induziu uma diminuição na hemoglobina dos militares e a suplementação não promoveu proteção contra este dano.
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Referências
Aguiló, A., Tauler, P., Sureda, A., Cases, N., Tur, J., & Pons, A. (2007). Antioxidant diet supplementation enhances aerobic performance in amateur sportsmen. Journal of Sports Sciences, 25(11), 1203–1210. https://doi.org/10.1080/02640410600951597
Ainsworth, B. E., Haskell, W. L., Herrmann, S. D., Meckes, N., Bassett, D. R., Tudor-Locke, C., Greer, J. L., Vezina, J., Whitt-Glover, M. C., & Leon, A. S. (2011). 2011 compendium of physical activities: A second update of codes and MET values. Medicine and Science in Sports and Exercise, 43(8), 1575–1581. https://doi.org/10.1249/MSS.0b013e31821ece12
Alis, R., Romagnoli, M., Primo-Carrau, C., Pareja-Galeano, H., Blesa, J. R., & Sanchis-Gomar, F. (2015). Effect of exhaustive running exercise on red blood cell distribution width. Clinical Chemistry and Laboratory Medicine, 53(2), e29–e31. https://doi.org/10.1515/cclm-2014-0749
Balushi, H. Al, Hannemann, A., Rees, D., Brewin, J., & Gibson, J. S. (2019). The effect of antioxidants on the properties of red blood cells from patients with sickle cell anemia. Frontiers in Physiology, 10(JUL). https://doi.org/10.3389/fphys.2019.00976
Barbosa, K. B. F., Costa, N. M. B., De Cássia Gonçalves Alfenas, R., De Paula, S. O., Minim, V. P. R., & Bressan, J. (2010). Estresse oxidativo: Conceito, implicações e fatores modulatórios. Revista de Nutricao, 23(4), 629–643. https://doi.org/10.1590/S1415-52732010000400013
Bissinger, R., Bhuyan, A. A. M., Qadri, S. M., & Lang, F. (2019). Oxidative stress, eryptosis and anemia: a pivotal mechanistic nexus in systemic diseases. FEBS Journal, 286(5), 826–854. https://doi.org/10.1111/febs.14606
Borg, G. (2000). Escalas de Borg para a dor e o esforço: percebido. São Paulo: Manole.
Brasil. (2013). Ministério da Defesa. Exército Brasileiro. Caderno de Instrução de Treinamento e Técnica Básica do Paaraquedista Militar - EB70-CI-11.001. 1a ed. file:///C:/Users/mabsm/Downloads/EB70-CI-11.001 (5).pdf
Brendel, H., Shahid, A., Hofmann, A., Mittag, J., Bornstein, S. R., Morawietz, H., & Brunssen, C. (2020). Nadph oxidase 4mediates the protective effects of physical activity against obesity-induced vascular dysfunction. Cardiovascular Research, 116(10), 1767–1778. https://doi.org/10.1093/cvr/cvz322
Chou, C. C., Sung, Y. C., Davison, G., Chen, C. Y., & Liao, Y. H. (2018). Short-term high-dose vitamin C and E supplementation attenuates muscle damage and inflammatory responses to repeated taekwondo competitions: A randomized placebo-controlled trial. International Journal of Medical Sciences, 15(11), 1217–1226. https://doi.org/10.7150/ijms.26340
Coates, A., Mountjoy, M., & Burr, J. (2017). Incidence of Iron Deficiency and Iron Deficient Anemia in Elite Runners and Triathletes. Clinical Journal of Sport Medicine, 27(5), 493–498. https://doi.org/10.1097/JSM.0000000000000390
Cotinguiba, G. G., Silva, J. ocksane do N., Azevedo, R. R. de S., Rocha, T. J. M., & Santos, A. F. dos. (2013). Método de Avaliação da Defesa Antioxidante : Uma Revisão de Literatura Methods of the Antioxidant Defense : A Literature Review. UNOPAR Cient., Ciênc. Biol. Saude, 15(3), 231–237.
Cunha, T. F., Bechara, L. R. G., Bacurau, A. V. N., Jannig, P. R., Voltarelli, V. A., Dourado, P. M., Vasconcelos, A. R., Scavone, C., Ferreira, J. C. B., & Brum, P. C. (2017). Exercise training decreases NADPH oxidase activity and restores skeletal muscle mass in heart failure rats. Journal of Applied Physiology, 122(4), 817–827. https://doi.org/10.1152/japplphysiol.00182.2016
Danese, E., Lippi, G., Sanchis-Gomar, F., Brocco, G., Rizzo, M., Banach, M., & Montagnana, M. (2017). Physical Exercise and DNA Injury: Good or Evil? In Advances in Clinical Chemistry (1st ed., Vol. 81). Elsevier Inc. https://doi.org/10.1016/bs.acc.2017.01.005
Dasso, N. A. (2019). How is exercise different from physical activity? A concept analysis. Nursing Forum, 54(1), 45–52. https://doi.org/10.1111/nuf.12296
Di Meo, S., Napolitano, G., & Venditti, P. (2019). Mediators of physical activity protection against ros-linked skeletal muscle damage. International Journal of Molecular Sciences, 20(12), 1–38. https://doi.org/10.3390/ijms20123024
Epstein, D., Borohovitz, A., Merdler, I., Furman, M., Atalli, E., Sorkin, A., Stainfeld, Y., Isenberg, Y., Mashiach, T., Shapira, S., Weisshof, R., & Dann, E. J. (2018). Prevalence of Iron Deficiency and Iron Deficiency Anemia in Strenuously Training Male Army Recruits. Acta Haematologica, 139(3), 141–147. https://doi.org/10.1159/000485736
Failace, R. (2015). Hemograma: manual de interpretação (Artmed Edi).
Fibach, E., & Rachmilewitz, E. (2008). Fibach, E., & Rachmilewitz, E. (2008). The role of oxidative stress in hemolytic anemia. Current molecular medicine, 8(7), 609-619.. 609–619.
Gammone, M. A., Gemello, E., Riccioni, G., & D’Orazio, N. (2014). Marine bioactives and potential application in sports. Marine Drugs, 12(5), 2357–2382. https://doi.org/10.3390/md12052357
Gaschler, M. M., & Stockwell, B. R. (2018). Lipid peroxidation in cell death. Biochem Biophys Res Commun, 176(1), 100–106. https://doi.org/10.1016/j.bbrc.2016.10.086.Lipid
Hasani, M., Djalalinia, S., Khazdooz, M., Asayesh, H., Zarei, M., Gorabi, A. M., Ansari, H., Qorbani, M., & Heshmat, R. (2019). Effect of selenium supplementation on antioxidant markers: a systematic review and meta-analysis of randomized controlled trials. Hormones, 18(4), 451–462. https://doi.org/10.1007/s42000-019-00143-3
Helmut Sies, Carsten Berndt, and D. P. J. (2017). Oxidative Stress. Annual Review of Biochemistry, 86, 715–748. https://doi.org/https://doi.org/10.1146/annurev-biochem- 061516-045037
Hoffbrand, A. V, & Steensma, D. P. (2020). Hoffbrand’s Essential Haematology (8 ed).
Kumar, D., & Rizvi, S. I. (2014). Markers of oxidative stress in senescent erythrocytes obtained from young and old age rats. Rejuvenation Research, 17(5), 446–452. https://doi.org/10.1089/rej.2014.1573
Lippi, G., & Sanchis-Gomar, F. (2019). Epidemiological, biological and clinical update on exercise-induced hemolysis. Annals of Translational Medicine, 7(12), 270–270. https://doi.org/10.21037/atm.2019.05.41
Lücker, A., Secomb, T. W., Weber, B., & Jenny, P. (2017). The relative influence of hematocrit and red blood cell velocity on oxygen transport from capillaries to tissue. Microcirculation, 24(3). https://doi.org/10.1111/micc.12337
Mairbäurl, H. (2013). Red blood cells in sports: Effects of exercise and training on oxygen supply by red blood cells. Frontiers in Physiology, 4 NOV(November), 1–13. https://doi.org/10.3389/fphys.2013.00332
Marengo, B., Nitti, M., Furfaro, A. L., Colla, R., Ciucis, C. De, Marinari, U. M., Pronzato, M. A., Traverso, N., & Domenicotti, C. (2016). Redox homeostasis and cellular antioxidant systems: Crucial players in cancer growth and therapy. Oxidative Medicine and Cellular Longevity, 2016. https://doi.org/10.1155/2016/6235641
Marfell-Jones, M., Esparza-Ros, F., & Vaquero-Cristóbal, R. (2019). ISAK accreditation handbook. Murcia: International Society for the Advancement of Kinanthropometry - ISAK.
Mariño, M. M., Grijota, F. J., Bartolomé, I., Siquier-Coll, J., Román, V. T., & Muñoz, D. (2020). Influence of physical training on erythrocyte concentrations of iron, phosphorus and magnesium. Journal of the International Society of Sports Nutrition, 17(1), 1–7. https://doi.org/10.1186/s12970-020-0339-y
Maynar, M., Bartolomé, I., Alves, J., Barrientos, G., Grijota, F. J., Robles, M. C., & Munõz, D. (2019). Influence of a 6-month physical training program on serum and urinary concentrations of trace metals in middle distance elite runners. Journal of the International Society of Sports Nutrition, 16(1), 1–10. https://doi.org/10.1186/s12970-019-0322-7
Mohanty, J. G., Nagababu, E., & Rifkind, J. M. (2014). Red blood cell oxidative stress impairs oxygen delivery and induces red blood cell aging. Frontiers in Physiology, 5 FEB(February), 1–6. https://doi.org/10.3389/fphys.2014.00084
Müller-Schilling, L., Gundlach, N., Böckelmann, I., & Sammito, S. (2019). Physical fitness as a risk factor for injuries and excessive stress symptoms during basic military training. International Archives of Occupational and Environmental Health, 92(6), 837–841. https://doi.org/10.1007/s00420-019-01423-6
Myhre, K. E., Webber, B. J., Cropper, T. L., Tchandja, J. N., Ahrendt, D. M., Dillon, C. A., Haas, R. W., Guy, S. L., Pawlak, M. T., & Federinko, S. P. (2016). Prevalence and Impact of Anemia on Basic Trainees in the US Air Force. Sports Medicine - Open, 2(1), 0–6. https://doi.org/10.1186/s40798-016-0047-y
Nikolaidis, P. T., Veniamakis, E., Rosemann, T., & Knechtle, B. (2018). Nutrition in ultra-endurance: State of the art. Nutrients, 10(12). https://doi.org/10.3390/nu10121995
Ojanen, T., Jalanko, P., & Kyröläinen, H. (2018). Physical fitness, hormonal, and immunological responses during prolonged military field training. Physiological Reports, 6(17), 1–10. https://doi.org/10.14814/phy2.13850
Padovani, R. M., Amaya-Farfán, J., Colugnati, F. A. B., & Domene, S. M. Á. (2006). Dietary reference intakes: Application of tables in nutritional studies. Revista de Nutricao, 19(6), 741–760. https://doi.org/10.1590/S1415-52732006000600010
Pingitore, A., Lima, G. P. P., Mastorci, F., Quinones, A., Iervasi, G., & Vassalle, C. (2015). Exercise and oxidative stress: Potential effects of antioxidant dietary strategies in sports. Nutrition, 31(7–8), 916–922. https://doi.org/10.1016/j.nut.2015.02.005
Pisoschi, A. M., & Pop, A. (2015). The role of antioxidants in the chemistry of oxidative stress: A review. European Journal of Medicinal Chemistry, 97, 55–74. https://doi.org/10.1016/j.ejmech.2015.04.040
Poblete-Aro, C., Russell-Guzmán, J., Parra, P., Soto-Muñoz, M., Villegas-González, B., Cofré-Bola-Dos, C., & Herrera-Valenzuela, T. (2018). Efecto del ejercicio físico sobre marcadores de estrés oxidativo en pacientes con diabetes mellitus tipo 2. Revista Médica de Chile, 146(3), 362–372. https://doi.org/10.4067/s0034-98872018000300362
Pollock, M., & Wilmore, J. (1993). Health and disease exercises: assessment and prescription for prevention and rehabilitation (2a ed).
Pospieszna, B., Kusy, K., Słomińska, E. M., Dudzinska, W., Ciekot-Sołtysiak, M., & Zieliński, J. (2020). The effect of training on erythrocyte energy status and plasma purine metabolites in athletes. Metabolites, 10(1), 1–15. https://doi.org/10.3390/metabo10010005
Rapido, F. (2017). The potential adverse effects of haemolysis. Blood Transfusion, 15(3), 218–221. https://doi.org/10.2450/2017.0311-16
Rifkind, J. M., Mohanty, J. G., & Nagababu, E. (2015). The pathophysiology of extracellular hemoglobin associated with enhanced oxidative reactions. Frontiers in Physiology, 6(JAN), 1–7. https://doi.org/10.3389/fphys.2014.00500
Saidi, K., Zouhal, H., Rhibi, F., Tijani, J. M., Boullosa, D., Chebbi, A., Hackney, A. C., Granacher, U., Bideau, B., & Abderrahman, A. Ben. (2019). Effects of a six-week period of congested match play on plasma volume variations, hematological parameters, training workload and physical fitness in elite soccer players. PLoS ONE, 14(7), 1–17. https://doi.org/10.1371/journal.pone.0219692
Sanchis-Gomar, F., Alis, R., Rodríguez-Vicente, G., Lucia, A., Casajús, J. A., & Garatachea, N. (2016). Blood and urinary abnormalities induced during and after 24-hour continuous running: A case report. Clinical Journal of Sport Medicine, 26(5), e100–e102. https://doi.org/10.1097/JSM.0000000000000222
Sies, H. (2018). On the history of oxidative stress: Concept and some aspects of current development. Current Opinion in Toxicology, 7(2018), 122–126. https://doi.org/10.1016/j.cotox.2018.01.002
Silva, A. C. da, Guimarães, T. T., Silva, J. M. da, Gomes, D. V., Mendonça, C. F., Pesquero, J. B., Palmisano, G., Moreira, J. C., & Pereira, M. D. (2021). Rabdomiólise em militares: uma missão de reconhecimento para prevenção. Military rhabdomyolysis: a reconnaissance mission for prevention. Jornal de Investigação Médica, 2(1).
Sumita, N. M., Andriolo, A., Ferreira, C. E. dos S., Campana, G. A., Oliveira, G., De, F., Brazão, F. V., Vasconcellos, L. de S., Junior, A. P., Lopes, A. C. W., Shcolnik, W., & Mendes, M. E. (2020). Sociedade Brasileira De Patologia Clínica / Medicina Laboratorial (SBPC/ML): Boas Práticas em Laboratório Clínico. In Medicina (Manole Ltd).
Szivak, T. K., Lee, E. C., Saenz, C., Flanagan, S. D., Focht, B. C., Volek, J. S., Maresh, C. M., & Kraemer, W. J. (2018). Adrenal stress and physical performance during military survival training. Aerospace Medicine and Human Performance, 89(2), 99–107. https://doi.org/10.3357/AMHP.4831.2018
Tarafdar, A., & Pula, G. (2018). The role of NADPH oxidases and oxidative stress in neurodegenerative disorders. International Journal of Molecular Sciences, 19(12). https://doi.org/10.3390/ijms19123824
Tsai, K. Z., Lai, S. W., Hsieh, C. J., Lin, C. S., Lin, Y. P., Tsai, S. C., Chung, P. S., Lin, Y. K., Lin, T. C., Ho, C. L., Han, C. L., Kwon, Y., Hsieh, C. B., & Lin, G. M. (2019). Association between mild anemia and physical fitness in a military male cohort: The CHIEF study. Scientific Reports, 9(1), 11165. https://doi.org/10.1038/s41598-019-47625-3
Valko, M., Jomova, K., Rhodes, C. J., Kuča, K., & Musílek, K. (2016). Redox- and non-redox-metal-induced formation of free radicals and their role in human disease. In Archives of Toxicology (Vol. 90, Issue 1). https://doi.org/10.1007/s00204-015-1579-5
Viña, J., Olaso-Gonzalez, G., Arc-Chagnaud, C., De La Rosa, A., & Gomez-Cabrera, M. C. (2020). Modulating Oxidant Levels to Promote Healthy Aging. Antioxidants and Redox Signaling, 33(8), 570–579. https://doi.org/10.1089/ars.2020.8036
WHO. (2020). guidelines on physical activity and sedentary behaviour. Geneva: World Health Organization; Licence: CC BY-NC-SA 3.0 IGO. https://www.who.int/publications/i/item/9789240015128.